Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression

doi: 10.1186/s13046-017-0499-z

Figure Lengend Snippet: MiR-381 is low-expressed in gastric cancer tissues and cell lines. a Bioinformatic analysis of the level of miR-381 expression of GSE26595 and GSE28700 datasets showed that miR-381 was significantly lower in gastric cancer tumors (T) than in non-tumor tissues (N). ( P < 0.01, P < 0.01, respectively); b QRT-PCR analysis of miR-381 expression in 103 pairs gastric cancer tissues and their corresponding adjacent non-tumor tissues. The expression of miRNA was normalized to U6 snRNA; c Relative miR-381 expression levels in gastric cancer tissues and adjacent non-tumor tissues. ** P < 0.01; d QRT-PCR analysis of miR-381 expression in gastric cancer cell lines (AGS, MKN-45, MKN-28, SGC-7901, BGC-823, MGC-803) and gastric epithelial cell line (GES-1). * P < 0.05, ** P < 0.01; e , f Overall survival and progression-free survival were compared between gastric cancer patients with low expression level of miR-381 and those with high level of miR-381

Article Snippet: The agomiR miR-381 (agomiR-381), antagomiR miR-381 (antagomiR-381) and their negative control (Con) Oligonucleotides were purchased from Shanghai GenePharma Co. Ltd.

Techniques: Expressing, Quantitative RT-PCR

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression

doi: 10.1186/s13046-017-0499-z

Figure Lengend Snippet: MiR-381 suppresses gastric cancer proliferation, migration and invasion in vitro. a , b Cell count and MTT assay analysis were used to evaluate the proliferation of gastric cancer cells (AGS and BGC-823) after transfection with the miR-381 agomiR (agomiR-381) or negative control (Con). ** P < 0.01. c , d Transwell assay was performed to detect the ability of migration and invasion of agomiR-381 transfected gastric cancer cells and their negative control. ** P < 0.01. ( e , f ) Cell count and MTT assay analysis were used to evaluate the proliferation of gastric cancer cells (MKN-28 and SGC-7901) after transfection with the miR-381 antagomiR (antagomiR-381) or negative control (Con). * P < 0.05, ** P < 0.01. g , h Transwell assay was performed to detect the ability of migration and invasion of antagomiR-381 transfected gastric cancer cells and their negative control. ** P < 0.01

Article Snippet: The agomiR miR-381 (agomiR-381), antagomiR miR-381 (antagomiR-381) and their negative control (Con) Oligonucleotides were purchased from Shanghai GenePharma Co. Ltd.

Techniques: Migration, In Vitro, Cell Counting, MTT Assay, Transfection, Negative Control, Transwell Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression

doi: 10.1186/s13046-017-0499-z

Figure Lengend Snippet: Overexpression of miR-381 inhibits gastric cancer growth and metastasis in vivo. a Growth curve of tumors in nude mice. Tumor diameters were measured every 3 days. ** P < 0.01. b The average weight of tumors in nude mice.** P < 0.01. c Representative HE staining of lung metastasis of agomiR-381 and negative control group. ** P < 0.01. Red arrows show the position of lung metastasis

Article Snippet: The agomiR miR-381 (agomiR-381), antagomiR miR-381 (antagomiR-381) and their negative control (Con) Oligonucleotides were purchased from Shanghai GenePharma Co. Ltd.

Techniques: Over Expression, In Vivo, Staining, Negative Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression

doi: 10.1186/s13046-017-0499-z

Figure Lengend Snippet: TMEM16A is a direct target of miR-381 in gastric cancer cells. a MiR-381 and its putative binding sequence in the wild-type and mutant 3′-UTR of TMEM16A. b Overexpression of miR-381 significantly decreased the luciferase activity that carried wild type (WT) but not mutant type (MUT) 3′-UTR of TMEM16A in gastric cancer cells. ** P < 0.01. c , d Overexpression of miR-381 markedly suppressed the mRNA and protein levels of TMEM16A in gastric cancer cells. ** P < 0.01. e Representative immunohistochemistry (IHC) staining of TMEM16A in high or low miR-381 expression gastric cancer tissues. f MiR-381 expression was inversely related to TMEM16A expression in gastric cancer tissues. ** P < 0.01. g The correlation between miR-381/TMEM16A co-expression and the percentage of metastatic lymph nodes, P < 0.05. h Overall survival of gastric cancer patients. Patient groups were separated based on expression status of miR-381 and TMEM16A

Article Snippet: The agomiR miR-381 (agomiR-381), antagomiR miR-381 (antagomiR-381) and their negative control (Con) Oligonucleotides were purchased from Shanghai GenePharma Co. Ltd.

Techniques: Binding Assay, Sequencing, Mutagenesis, Over Expression, Luciferase, Activity Assay, Immunohistochemistry, Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression

doi: 10.1186/s13046-017-0499-z

Figure Lengend Snippet: TMEM16A mediates the effects of miR-381 on migration and invasion in gastric cancer cells. Gastric cancer cells tranfected with agomiR-381 and negative control, together with blank vector or TMEM16A overexpression plasmid. a Western blot analysis showed that ectopic expression of miR-381 reduced the TMEM16A protein expression, while co-transfection of TMEM16A overexpression TMEM16A plasmids could recover the TMEM16A expression. ** P < 0.01. b , c Co-transfection of TMEM16A overexpression reverted the suppressive effects of miR-381 overexpression on the migration and invasion of gastric cancer cells** P < 0.01. d , e TMEM16A-overexpressing did not reverse the inhibition of gastric cancer cell proliferation induced by the miR-381 overexpression

Article Snippet: The agomiR miR-381 (agomiR-381), antagomiR miR-381 (antagomiR-381) and their negative control (Con) Oligonucleotides were purchased from Shanghai GenePharma Co. Ltd.

Techniques: Migration, Negative Control, Plasmid Preparation, Over Expression, Western Blot, Expressing, Cotransfection, Inhibition

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression

doi: 10.1186/s13046-017-0499-z

Figure Lengend Snippet: Mir-381 suppresses TGF-β signaling pathway and down-regulated EMT phenotypes. Gastric cancer cells tranfected with anomiR-381 and negative control, together with blank vector or TMEM16A overexpression plasmid. a ELISA assay indicated that supernatant concentrations of TGF-β1 and TGF-β2 dramatically reduced in agomiR-381 group, and overexpression of TMEM16A could partially rescue their expression. ** P < 0.01. b QRT-PCR assay showed that mRNA of TGF-βs decreased in agomiR-381 group, which not be affected by overexpression of TMEM16A. c Protein expression of TGF-β1 and TGF-β2 in gastric cancer cells down-regulated in miR-381 overexpression group, and further decreased in TMEM16A overexpression group. d , e Transwell assay showed that the effect of miR-381 on gastric cancer cells migration and invasion could be partially recovered by supplement of TGF-β. ** P < 0.01. f Western blot assay was employed to detect the expression of EMT related markers in gastric cancer cells

Article Snippet: The agomiR miR-381 (agomiR-381), antagomiR miR-381 (antagomiR-381) and their negative control (Con) Oligonucleotides were purchased from Shanghai GenePharma Co. Ltd.

Techniques: Negative Control, Plasmid Preparation, Over Expression, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Transwell Assay, Migration, Western Blot

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: MicroRNA-381 inhibits the metastasis of gastric cancer by targeting TMEM16A expression

doi: 10.1186/s13046-017-0499-z

Figure Lengend Snippet: Model for miR-381-mediated inhibition of cell proliferation and migration via its regulation of the TMEM16A and TGF-β signaling pathway. CDS coding sequence, 3′UTR 3′-untranslated region, EMT epithelial-mesenchymal transition

Article Snippet: The agomiR miR-381 (agomiR-381), antagomiR miR-381 (antagomiR-381) and their negative control (Con) Oligonucleotides were purchased from Shanghai GenePharma Co. Ltd.

Techniques: Inhibition, Migration, Sequencing